6O81

Electron cryo-microscopy of the eukaryotic translation initiation factor 2B bound to translation initiation factor 2 from Homo sapiens

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Translation initiation factor eIF-2B subunit epsilon	polymer	721	80366.5	2	UniProt (Q13144) Pfam (PF02020) In PDB	Homo sapiens (Human)	eIF-2B GDP-GTP exchange factor subunit epsilon
2	C, D	Translation initiation factor eIF-2B subunit beta	polymer	368	41008.6	2	UniProt (P49770) Pfam (PF01008) In PDB	Homo sapiens (Human)	S20I15,S20III15,eIF-2B GDP-GTP exchange factor subunit beta
3	E, F	Translation initiation factor eIF-2B subunit delta	polymer	523	57640.2	2	UniProt (Q9UI10) Pfam (PF01008) In PDB	Homo sapiens (Human)	eIF-2B GDP-GTP exchange factor subunit delta
4	G, H	Translation initiation factor eIF-2B subunit alpha	polymer	305	33754.1	2	UniProt (Q14232) Pfam (PF01008) In PDB	Homo sapiens (Human)	eIF-2B GDP-GTP exchange factor subunit alpha
5	I, J	Translation initiation factor eIF-2B subunit gamma	polymer	452	50304.2	2	UniProt (Q9NR50) Pfam (PF00483) In PDB	Homo sapiens (Human)	eIF-2B GDP-GTP exchange factor subunit gamma
6	L, M	Eukaryotic translation initiation factor 2 subunit 1	polymer	315	36161.2	2	UniProt (P05198) Pfam (PF00575) Pfam (PF07541) In PDB	Homo sapiens (Human)	Eukaryotic translation initiation factor 2 subunit alpha,eIF-2alpha
7	S, T	Eukaryotic translation initiation factor 2 subunit 3	polymer	472	51150.4	2	UniProt (P41091) Pfam (PF09173) Pfam (PF00009) Pfam (PF03144) In PDB	Homo sapiens (Human)	Eukaryotic translation initiation factor 2 subunit gamma X,eIF-2gX
8	d, e	Translation initiation factor eiF2 beta-subunit	polymer	14	1209.5	2		Homo sapiens (human)
9	C	2-(4-chloranylphenoxy)-~{N}-[4-[2-(4-chloranylphenoxy)ethanoylamino]cyclohexyl]ethanamide	non-polymer		451.3	1	Chemie (C7B)

Sequence modifications

A, B: 1 - 721 (UniProt: Q13144)

PDB	External Database	Details
Lys 563	Arg 563	conflict
Gly 678	Glu 678	conflict

C, D: 2 - 351 (UniProt: P49770)

PDB	External Database	Details
Met -16	-	initiating methionine
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Ser 1	-	expression tag

S, T: 1 - 472 (UniProt: P41091)

PDB	External Database	Details
Ala 304	Asp 304	conflict
Lys 314	Ile 314	conflict

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	703189.5
Non-Polymers*	Number of molecules	1
	Total formula weight	451.3
All*	Total formula weight	703640.8

*Water molecules are not included.