6O7H
Cryo-EM structure of Csm-crRNA-target RNA ternary complex in complex with cA4 in type III-A CRISPR-Cas system
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) | polymer | 791 | 89750.6 | 1 | UniProt (B6YWB8) Pfam (PF01966) Pfam (PF18211) Pfam (PF22335) | Thermococcus onnurineus (strain NA1) | ssDNase Cas10,Cyclic oligoadenylate synthase,ToCsm1 |
| 2 | B (B) | Csm2 | polymer | 188 | 21235.3 | 1 | UniProt (B6YWB9) Pfam (PF03750) | Thermococcus onnurineus (strain NA1) | |
| 3 | C, D (C, D) | Csm3 | polymer | 292 | 32822.1 | 2 | UniProt (B6YWC0) Pfam (PF03787) | Thermococcus onnurineus (strain NA1) | |
| 4 | E (E) | Csm4 | polymer | 289 | 32345.1 | 1 | UniProt (B6YWC1) | Thermococcus onnurineus (strain NA1) | |
| 5 | F (G) | RNA (38-MER) | polymer | 38 | 12463.4 | 1 | Thermococcus onnurineus (strain NA1) | ||
| 6 | G (H) | RNA (40-MER) | polymer | 40 | 12750.7 | 1 | Thermococcus onnurineus (strain NA1) | ||
| 7 | H (F) | Csm5 | polymer | 378 | 40588.4 | 1 | Pfam (PF03787) UniProt (by SIFTS) (B6YWC2) | Thermococcus onnurineus (strain NA1) | |
| 8 | I (K) | Cyclic DNA cA4 | polymer | 4 | 1271.9 | 1 | synthetic construct | DNA (5'-R(P*AP*AP*AP*A)-3') | |
| 9 | J, K, L (A, C, D) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) | |||
| 10 | M (H) | GUANOSINE-5'-MONOPHOSPHATE | non-polymer | 363.2 | 1 | Chemie (5GP) |
Sequence modifications
A: 1 - 777 (UniProt: B6YWB8)
B: 2 - 186 (UniProt: B6YWB9)
C, D: 1 - 290 (UniProt: B6YWC0)
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | initiating methionine |
| Gly -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Ser -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Pro 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Val 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 36 | Asp 36 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 276049.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 559.4 | |
| All* | Total formula weight | 276608.9 |
