6O7E

Cryo-EM structure of Csm-crRNA-target RNA ternary complex in complex with AMPPNP in type III-A CRISPR-Cas system

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Csm1	polymer	791	89682.5	1	UniProt (B6YWB8) Pfam (PF01966) Pfam (PF18211) Pfam (PF22335) In PDB	Thermococcus onnurineus (strain NA1)
2	B	Csm2	polymer	187	21210.3	1	UniProt (B6YWB9) Pfam (PF03750) In PDB	Thermococcus onnurineus (strain NA1)
3	C, D	Csm3	polymer	291	32809.0	2	UniProt (B6YWC0) Pfam (PF03787) In PDB	Thermococcus onnurineus (strain NA1)
4	E	Csm4	polymer	289	32345.1	1	UniProt (B6YWC1) In PDB	Thermococcus onnurineus (strain NA1)
5	G	RNA (38-MER)	polymer	38	12463.4	1		Thermococcus onnurineus
6	H	RNA (40-MER)	polymer	40	12750.7	1		Thermococcus onnurineus
7	Y	Csm5	polymer	378	40588.4	1	Pfam (PF03787) UniProt (by SIFTS) (B6YWC2) In PDB	Thermococcus onnurineus NA1
8	A, C, D	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
9	A	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
10	A	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)

Sequence modifications

A: 1 - 777 (UniProt: B6YWB8)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Ala 14	His 14	engineered mutation
Asn 15	Asp 15	engineered mutation

B: 1 - 186 (UniProt: B6YWB9)

PDB	External Database	Details
Ser 0	-	expression tag

C, D: 1 - 290 (UniProt: B6YWC0)

PDB	External Database	Details
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	274658.4
Non-Polymers*	Number of molecules	8
	Total formula weight	1373.4
All*	Total formula weight	276031.9

*Water molecules are not included.