6O75

Crystal structure of Csm1-Csm4 cassette in complex with pppApA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)	polymer	791	89706.6	1	UniProt (B6YWB8) Pfam (PF01966) Pfam (PF18211) Pfam (PF22335)	Thermococcus onnurineus	ssDNase Cas10,Cyclic oligoadenylate synthase,ToCsm1
2	B (B)	Csm4	polymer	289	32345.1	1	UniProt (B6YWC1)	Thermococcus onnurineus
3	C, D (C, D)	RNA (5'-D(*(ATP))-R(P*A)-3')	polymer	2	773.4	2		synthetic construct
4	E, F (A)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)

Sequence modifications

A: 1 - 777 (UniProt: B6YWB8)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Ala 589	Asp 589	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	123598.5
Non-Polymers*	Number of molecules	2
	Total formula weight	109.9
All*	Total formula weight	123708.4

*Water molecules are not included.