6NYA

Crystal Structure of ubiquitin E1 (Uba1) in complex with Ubc3 (Cdc34) and ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Ubiquitin-activating enzyme E1 1	polymer	1017	113704.2	2	UniProt (P22515) Pfam (PF00899) Pfam (PF16190) Pfam (PF16191) Pfam (PF10585) Pfam (PF09358)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
2	B, E (B, E)	Ubiquitin	polymer	85	9821.1	2	UniProt (A0A1D6RLC6) Pfam (PF00240) UniProt (by SIFTS) (P69326)	Triticum aestivum (Wheat)
3	C, F (C, F)	Ubiquitin-conjugating enzyme E2-34 kDa	polymer	197	22427.2	2	UniProt (P14682) Pfam (PF00179)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cell division control protein 34,E2 ubiquitin-conjugating enzyme 3,E3 ubiquitin ligase complex SCF subunit CDC34,Ubiquitin carrier protein,Ubiquitin-protein ligase
4	DA, G (D, A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
5	CA, H (D, A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	EA, FA, GA, HA, I... (D, A)	SULFATE ION	non-polymer		96.1	15	Chemie (SO4)
7	AA, BA, LA, MA, NA... (A, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	21	Chemie (EDO)
8	UA, VA, WA, XA, YA... (A, B, C, D, E...)	water	water		18.0	909	Chemie (HOH)

Sequence modifications

A, D: 11 - 1024 (UniProt: P22515)

PDB	External Database	Details
Gly 8	-	expression tag
Ala 9	-	expression tag
Met 10	-	expression tag

B, E: 1 - 76 (UniProt: A0A1D6RLC6)

PDB	External Database	Details
Met -8	-	initiating methionine
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Arg 6	Lys 82	engineered mutation
Arg 11	Lys 87	engineered mutation
Arg 27	Lys 103	engineered mutation
Arg 29	Lys 105	engineered mutation
Arg 33	Lys 109	engineered mutation
Arg 48	Lys 124	engineered mutation
Arg 63	Lys 139	engineered mutation

C, F: 3 - 195 (UniProt: P14682)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Met 1	-	expression tag
Ala 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	291904.9
Non-Polymers*	Number of molecules	40
	Total formula weight	3807.3
All*	Total formula weight	295712.2

*Water molecules are not included.