6NXC

ECAI(T162A) MUTANT IN COMPLEX WITH CITRATE AT PH 4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	L-asparaginase 1	polymer	357	39190.3	4	UniProt (P0A962) Pfam (PF00710) Pfam (PF17763)	Escherichia coli (strain K12)	L-asparaginase I,L-ASNase I,L-asparagine amidohydrolase I
2	DA, E, N, X (D, A, B, C)	CITRIC ACID	non-polymer		192.1	4	Chemie (CIT)
3	F, G, GA, HA, K... (A, D, B, C)	1,2-ETHANEDIOL	non-polymer		62.1	11	Chemie (EDO)
4	AA, BA, EA, FA, H... (C, D, A, B)	CHLORIDE ION	non-polymer		35.5	10	Chemie (CL)
5	I (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	CA, M, O, W (D, A, B, C)	ASPARAGINE	non-polymer		132.1	4	Chemie (ASN)
7	IA, JA, KA, LA (A, B, C, D)	water	water		18.0	565	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 337 (UniProt: P0A962)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 162	Thr 162	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	156761.4
Non-Polymers*	Number of molecules	30
	Total formula weight	2426.3
All*	Total formula weight	159187.7

*Water molecules are not included.