6NXC
ECAI(T162A) MUTANT IN COMPLEX WITH CITRATE AT PH 4
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | L-asparaginase 1 | polymer | 357 | 39190.3 | 4 | UniProt (P0A962) Pfam (PF00710) Pfam (PF17763) In PDB | Escherichia coli (strain K12) | L-asparaginase I,L-ASNase I,L-asparagine amidohydrolase I |
2 | D, A, B, C | CITRIC ACID | non-polymer | 192.1 | 4 | Chemie (CIT) | |||
3 | A, D, B, C | 1,2-ETHANEDIOL | non-polymer | 62.1 | 11 | Chemie (EDO) | |||
4 | C, D, A, B | CHLORIDE ION | non-polymer | 35.5 | 10 | Chemie (CL) | |||
5 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
6 | D, A, B, C | ASPARAGINE | non-polymer | 132.1 | 4 | Chemie (ASN) | |||
7 | water | water | 18.0 | 565 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 337 (UniProt: P0A962)
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Ala 162 | Thr 162 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 156761.4 | |
Non-Polymers* | Number of molecules | 30 |
Total formula weight | 2426.3 | |
All* | Total formula weight | 159187.7 |