6NXC
ECAI(T162A) MUTANT IN COMPLEX WITH CITRATE AT PH 4
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004067 | molecular_function | asparaginase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006520 | biological_process | amino acid metabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0033345 | biological_process | asparagine catabolic process via L-aspartate |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0051289 | biological_process | protein homotetramerization |
| B | 0004067 | molecular_function | asparaginase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006520 | biological_process | amino acid metabolic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0033345 | biological_process | asparagine catabolic process via L-aspartate |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0051289 | biological_process | protein homotetramerization |
| C | 0004067 | molecular_function | asparaginase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006520 | biological_process | amino acid metabolic process |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0033345 | biological_process | asparagine catabolic process via L-aspartate |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0051289 | biological_process | protein homotetramerization |
| D | 0004067 | molecular_function | asparaginase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006520 | biological_process | amino acid metabolic process |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0033345 | biological_process | asparagine catabolic process via L-aspartate |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0051289 | biological_process | protein homotetramerization |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 11 |
| Details | binding site for residue CIT A 9101 |
| Chain | Residue |
| A | GLY13 |
| A | HOH9248 |
| C | ASN246 |
| A | THR14 |
| A | ASP59 |
| A | SER60 |
| A | SER61 |
| A | GLY90 |
| A | THR91 |
| A | ASP92 |
| A | SER117 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | binding site for residue EDO A 9102 |
| Chain | Residue |
| A | HIS52 |
| A | ASP74 |
| A | HIS78 |
| site_id | AC3 |
| Number of Residues | 10 |
| Details | binding site for residue EDO A 9103 |
| Chain | Residue |
| A | ASN176 |
| A | THR271 |
| A | CYS273 |
| A | MET274 |
| A | SER275 |
| A | GLY276 |
| A | ASP299 |
| A | MET300 |
| A | THR301 |
| A | ASN9109 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue CL A 9104 |
| Chain | Residue |
| A | HIS165 |
| A | ALA166 |
| A | ASP167 |
| C | SER275 |
| site_id | AC5 |
| Number of Residues | 9 |
| Details | binding site for residue GOL A 9105 |
| Chain | Residue |
| A | ASP92 |
| A | GLN118 |
| A | LYS163 |
| A | ALA166 |
| A | HOH9201 |
| B | ILE185 |
| C | GLY243 |
| C | VAL244 |
| C | ASN246 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | binding site for residue CL A 9106 |
| Chain | Residue |
| A | ASN107 |
| A | GLY198 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue EDO A 9107 |
| Chain | Residue |
| A | ARG240 |
| A | GLN272 |
| C | TYR96 |
| C | ARG240 |
| C | VAL302 |
| C | ASN402 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue CL A 9108 |
| Chain | Residue |
| A | SER275 |
| A | GLY276 |
| C | HIS165 |
| C | ALA166 |
| C | ASP167 |
| site_id | AC9 |
| Number of Residues | 12 |
| Details | binding site for residue ASN A 9109 |
| Chain | Residue |
| A | ALA162 |
| A | ARG240 |
| A | THR271 |
| A | GLN272 |
| A | CYS273 |
| A | THR301 |
| A | VAL302 |
| A | GLU303 |
| A | EDO9103 |
| A | HOH9252 |
| C | ARG240 |
| C | EDO401 |
| site_id | AD1 |
| Number of Residues | 12 |
| Details | binding site for residue ASN B 9102 |
| Chain | Residue |
| B | ALA162 |
| B | ARG240 |
| B | THR271 |
| B | GLN272 |
| B | CYS273 |
| B | THR301 |
| B | VAL302 |
| B | GLU303 |
| B | EDO9104 |
| B | HOH9216 |
| B | HOH9233 |
| D | ARG240 |
| site_id | AD2 |
| Number of Residues | 7 |
| Details | binding site for residue EDO B 9103 |
| Chain | Residue |
| B | HIS0 |
| B | GLN2 |
| B | LYS3 |
| B | PRO44 |
| B | GLU45 |
| B | MET46 |
| B | CL9106 |
| site_id | AD3 |
| Number of Residues | 10 |
| Details | binding site for residue EDO B 9104 |
| Chain | Residue |
| B | ASN176 |
| B | THR271 |
| B | CYS273 |
| B | MET274 |
| B | SER275 |
| B | GLY276 |
| B | ASP299 |
| B | MET300 |
| B | THR301 |
| B | ASN9102 |
| site_id | AD4 |
| Number of Residues | 8 |
| Details | binding site for residue EDO B 9105 |
| Chain | Residue |
| A | ILE185 |
| B | ASP92 |
| B | GLN118 |
| B | LYS163 |
| B | ALA166 |
| D | GLY243 |
| D | VAL244 |
| D | ASN246 |
| site_id | AD5 |
| Number of Residues | 4 |
| Details | binding site for residue CL B 9106 |
| Chain | Residue |
| B | LYS4 |
| B | PRO47 |
| B | EDO9103 |
| B | HOH9250 |
| site_id | AD6 |
| Number of Residues | 4 |
| Details | binding site for residue CL B 9107 |
| Chain | Residue |
| B | ALA166 |
| B | ASP167 |
| D | SER275 |
| D | HOH654 |
| site_id | AD7 |
| Number of Residues | 4 |
| Details | binding site for residue CL B 9108 |
| Chain | Residue |
| B | SER275 |
| D | HIS165 |
| D | ALA166 |
| D | ASP167 |
| site_id | AD8 |
| Number of Residues | 7 |
| Details | binding site for residue EDO C 401 |
| Chain | Residue |
| A | TYR96 |
| A | ARG240 |
| A | VAL302 |
| A | ASN9109 |
| C | THR216 |
| C | ARG240 |
| C | GLN272 |
| site_id | AD9 |
| Number of Residues | 11 |
| Details | binding site for residue ASN C 402 |
| Chain | Residue |
| A | ARG240 |
| A | EDO9107 |
| C | ALA162 |
| C | ARG240 |
| C | THR271 |
| C | CYS273 |
| C | THR301 |
| C | VAL302 |
| C | GLU303 |
| C | EDO404 |
| C | HOH524 |
| site_id | AE1 |
| Number of Residues | 9 |
| Details | binding site for residue EDO C 404 |
| Chain | Residue |
| C | ASN176 |
| C | THR271 |
| C | MET274 |
| C | SER275 |
| C | GLY276 |
| C | ASP299 |
| C | MET300 |
| C | THR301 |
| C | ASN402 |
| site_id | AE2 |
| Number of Residues | 7 |
| Details | binding site for residue EDO C 405 |
| Chain | Residue |
| A | GLY243 |
| A | VAL244 |
| A | ASN246 |
| C | ASP92 |
| C | GLN118 |
| C | LYS163 |
| C | ALA166 |
| site_id | AE3 |
| Number of Residues | 3 |
| Details | binding site for residue CL C 406 |
| Chain | Residue |
| C | GLN69 |
| C | HIS70 |
| C | GLU73 |
| site_id | AE4 |
| Number of Residues | 1 |
| Details | binding site for residue CL D 401 |
| Chain | Residue |
| D | ARG33 |
| site_id | AE5 |
| Number of Residues | 12 |
| Details | binding site for residue ASN D 402 |
| Chain | Residue |
| B | ARG240 |
| D | ALA162 |
| D | ARG240 |
| D | THR271 |
| D | GLN272 |
| D | CYS273 |
| D | THR301 |
| D | VAL302 |
| D | GLU303 |
| D | EDO406 |
| D | HOH542 |
| D | HOH554 |
| site_id | AE6 |
| Number of Residues | 3 |
| Details | binding site for residue CL D 404 |
| Chain | Residue |
| D | LYS4 |
| D | PRO47 |
| D | HOH544 |
| site_id | AE7 |
| Number of Residues | 1 |
| Details | binding site for residue CL D 405 |
| Chain | Residue |
| D | GLU201 |
| site_id | AE8 |
| Number of Residues | 9 |
| Details | binding site for residue EDO D 406 |
| Chain | Residue |
| D | ASN176 |
| D | THR271 |
| D | CYS273 |
| D | MET274 |
| D | SER275 |
| D | GLY276 |
| D | ASP299 |
| D | MET300 |
| D | ASN402 |
| site_id | AE9 |
| Number of Residues | 8 |
| Details | binding site for residue EDO D 407 |
| Chain | Residue |
| B | GLY243 |
| B | VAL244 |
| B | ASN246 |
| C | ILE185 |
| D | ASP92 |
| D | GLN118 |
| D | LYS163 |
| D | ALA166 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: O-isoaspartyl threonine intermediate => ECO:0000255|PROSITE-ProRule:PRU10099, ECO:0000255|PROSITE-ProRule:PRU10100, ECO:0000269|PubMed:17451745 |
| Chain | Residue | Details |
| A | THR14 | |
| B | THR14 | |
| C | THR14 | |
| D | THR14 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 20 |
| Details | BINDING: BINDING => ECO:0007744|PDB:2P2N |
| Chain | Residue | Details |
| A | ASP59 | |
| B | THR271 | |
| C | ASP59 | |
| C | THR91 | |
| C | ALA162 | |
| C | ARG240 | |
| C | THR271 | |
| D | ASP59 | |
| D | THR91 | |
| D | ALA162 | |
| D | ARG240 | |
| A | THR91 | |
| D | THR271 | |
| A | ALA162 | |
| A | ARG240 | |
| A | THR271 | |
| B | ASP59 | |
| B | THR91 | |
| B | ALA162 | |
| B | ARG240 |
