6NX7
ECAII(D90T,K162T) MUTANT IN COMPLEX WITH CITRATE AT PH 5.6
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | L-asparaginase 2 | polymer | 333 | 35544.8 | 2 | UniProt (P00805) Pfam (PF00710) Pfam (PF17763) | Escherichia coli (strain K12) | L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II |
| 2 | C (A) | ACETIC ACID | non-polymer | 60.1 | 1 | Chemie (ACY) | |||
| 3 | D (B) | CITRIC ACID | non-polymer | 192.1 | 1 | Chemie (CIT) | |||
| 4 | E, F (A, B) | water | water | 18.0 | 311 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 326 (UniProt: P00805)
| PDB | External Database | Details |
|---|---|---|
| Met -6 | - | initiating methionine |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| Thr 90 | Asp 112 | conflict |
| Thr 162 | Lys 184 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 71089.6 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 252.2 | |
| All* | Total formula weight | 71341.8 |
