6NX6

ECAII(D90T,K162T) MUTANT IN COMPLEX WITH CITRATE AT PH 5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	L-asparaginase 2	polymer	333	35544.8	2	UniProt (P00805) Pfam (PF00710) Pfam (PF17763) In PDB	Escherichia coli (strain K12)	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	A	ACETIC ACID	non-polymer		60.1	1	Chemie (ACY)
3	B	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)
4		water	water		18.0	288	Chemie (HOH)

Sequence modifications

A, B: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Thr 90	Asp 112	engineered mutation
Thr 162	Lys 184	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	71089.6
Non-Polymers*	Number of molecules	2
	Total formula weight	252.2
All*	Total formula weight	71341.8

*Water molecules are not included.