6NT4

Cryo-EM structure of a human-cockroach hybrid Nav channel bound to alpha-scorpion toxin AaH2.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Sodium channel protein PaFPC1,Sodium channel protein type 9 subunit alpha,Sodium channel protein PaFPC1	polymer	1559	178763.7	1	UniProt (D0E0C2) UniProt (Q15858) Pfam (PF00520) Pfam (PF08016) Pfam (PF16905) In PDB	Periplaneta americana (American cockroach)	PaFPC1,NavPaS,Neuroendocrine sodium channel,hNE-Na,Peripheral sodium channel 1,PN1,Sodium channel protein type IX subunit alpha,Voltage-gated sodium channel subunit alpha Nav1.7,PaFPC1,NavPaS
2	B, C	Alpha-mammal toxin AaH2	polymer	65	7260.2	2	UniProt (P01484) Pfam (PF00537) In PDB	Androctonus australis (Sahara scorpion)	AaH II,AaHII,Neurotoxin 2
3	D	beta-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G61217QH)
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)
5	A	(7E,21R,24S)-27-amino-24-hydroxy-18,24-dioxo-19,23,25-trioxa-24lambda~5~-phosphaheptacos-7-en-21-yl (9Z,12E)-octadeca-9,12-dienoate	non-polymer		742.0	5	Chemie (76F)
6	A	Digitonin	non-polymer		1229.3	1	Chemie (AJP)
7	A	1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE	non-polymer		723.0	1	Chemie (LHG)
8	A	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	1	Chemie (Y01)

Sequence modifications

A: 1 - 1154 (UniProt: D0E0C2)

PDB	External Database	Details
Trp -53	-	expression tag
Ser -52	-	expression tag
His -51	-	expression tag
Pro -50	-	expression tag
Gln -49	-	expression tag
Phe -48	-	expression tag
Glu -47	-	expression tag
Lys -46	-	expression tag
Gly -45	-	expression tag
Gly -44	-	expression tag
Gly -43	-	expression tag
Ser -42	-	expression tag
Gly -41	-	expression tag
Gly -40	-	expression tag
Gly -39	-	expression tag
Ser -38	-	expression tag
Gly -37	-	expression tag
Gly -36	-	expression tag
Ser -35	-	expression tag
Ala -34	-	expression tag
Trp -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
Pro -30	-	expression tag
Gln -29	-	expression tag
Phe -28	-	expression tag
Glu -27	-	expression tag
Lys -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Ser -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Asp -20	-	expression tag
Tyr -19	-	expression tag
Lys -18	-	expression tag
Asp -17	-	expression tag
Asp -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Lys -13	-	expression tag
Gly -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Ser 270	Phe 270	conflict
Leu 274	Val 274	conflict
Ile 275	Leu 275	conflict
Leu 279	Ile 279	conflict
Phe 280	Tyr 280	conflict
Asn 283	Val 283	conflict
Lys 285	Thr 285	conflict
His 286	Gln 286	conflict

A: 1155 - 1286 (UniProt: Q15858)
A: 1287 - 1505 (UniProt: D0E0C2)
B, C: 1 - 64 (UniProt: P01484)

PDB	External Database	Details
Nh2 65	-	amidation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	193284.0
Branched	Number of molecules	1
	Total formula weight	910.8
Non-Polymers*	Number of molecules	13
	Total formula weight	7255.1
All*	Total formula weight	201450.0

*Water molecules are not included.