6NT3

Cryo-EM structure of a human-cockroach hybrid Nav channel.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Sodium channel protein type 9 subunit alpha, Sodium channel protein PaFPC1, chimeric construct	polymer	1559	178763.7	1	UniProt (D0E0C2) UniProt (Q15858) Pfam (PF00520) Pfam (PF08016) Pfam (PF16905)	Periplaneta americana (American cockroach)	PaFPC1,NavPaS,Neuroendocrine sodium channel,hNE-Na,Peripheral sodium channel 1,PN1,Sodium channel protein type IX subunit alpha,Voltage-gated sodium channel subunit alpha Nav1.7,PaFPC1,NavPaS
2	B (B)	beta-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G61217QH)
3	C, D (C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	E, F, G, H (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
5	I (A)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	1	Chemie (Y01)
6	J, K, L, M (A)	(7E,21R,24S)-27-amino-24-hydroxy-18,24-dioxo-19,23,25-trioxa-24lambda~5~-phosphaheptacos-7-en-21-yl (9Z,12E)-octadeca-9,12-dienoate	non-polymer		742.0	4	Chemie (76F)
7	N (A)	1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE	non-polymer		723.0	1	Chemie (LHG)
8	O (A)	Digitonin	non-polymer		1229.3	1	Chemie (AJP)

Sequence modifications

A: 1 - 1154 (UniProt: D0E0C2)

PDB	External Database	Details
Trp -53	-	expression tag
Ser -52	-	expression tag
His -51	-	expression tag
Pro -50	-	expression tag
Gln -49	-	expression tag
Phe -48	-	expression tag
Glu -47	-	expression tag
Lys -46	-	expression tag
Gly -45	-	expression tag
Gly -44	-	expression tag
Gly -43	-	expression tag
Ser -42	-	expression tag
Gly -41	-	expression tag
Gly -40	-	expression tag
Gly -39	-	expression tag
Ser -38	-	expression tag
Gly -37	-	expression tag
Gly -36	-	expression tag
Ser -35	-	expression tag
Ala -34	-	expression tag
Trp -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
Pro -30	-	expression tag
Gln -29	-	expression tag
Phe -28	-	expression tag
Glu -27	-	expression tag
Lys -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Ser -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Asp -20	-	expression tag
Tyr -19	-	expression tag
Lys -18	-	expression tag
Asp -17	-	expression tag
Asp -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Lys -13	-	expression tag
Gly -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Ser 270	Phe 270	conflict
Leu 274	Val 274	conflict
Ile 275	Leu 275	conflict
Leu 279	Ile 279	conflict
Phe 280	Tyr 280	conflict
Asn 283	Val 283	conflict
Lys 285	Thr 285	conflict
His 286	Gln 286	conflict

A: 1155 - 1286 (UniProt: Q15858)
A: 1287 - 1505 (UniProt: D0E0C2)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	178763.7
Branched	Number of molecules	3
	Total formula weight	1759.6
Non-Polymers*	Number of molecules	11
	Total formula weight	6291.9
All*	Total formula weight	186815.2

*Water molecules are not included.