6NQJ

Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Fluorescent protein Dronpa	polymer	257	28961.6	4	UniProt (Q5TLG6) Pfam (PF01353)	Echinophyllia sp. SC22
2	E, F, G, H (A, B, C, D)	water	water		18.0	642	Chemie (HOH)

Sequence modifications

A, B, C, D: 3 - 223 (UniProt: Q5TLG6)

PDB	External Database	Details
Gly -27	-	expression tag
Ser -26	-	expression tag
Ser -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
Gly -16	-	expression tag
Leu -15	-	expression tag
Val -14	-	expression tag
Pro -13	-	expression tag
Gly -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
Met -8	-	expression tag
Val -7	-	expression tag
Ser -6	-	expression tag
Lys -5	-	expression tag
Gly -4	-	expression tag
Glu -3	-	expression tag
Glu -2	-	expression tag
Asn -1	-	expression tag
Asn 0	-	expression tag
Met 1	-	expression tag
Ala 2	-	expression tag
Gyc 63	Cys 62	chromophore
Gyc 63	Tyr 63	chromophore
Gyc 63	Gly 64	chromophore
Thr 159	Met 159	engineered mutation
Gly 218	Glu 218	engineered mutation
Met 224	-	expression tag
Asp 225	-	expression tag
Glu 226	-	expression tag
Leu 227	-	expression tag
Tyr 228	-	expression tag
Lys 229	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	115846.3
All*	Total formula weight	115846.3

*Water molecules are not included.