6NQJ

Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	70.030, 85.795, 78.582
Unit cell angles	90.00, 110.11, 90.00

Refinement procedure

Resolution	38.280 - 2.000
R-factor	0.177
Rwork	0.174
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uts
RMSD bond length	0.007
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.281	2.050
High resolution limit [Å]	2.000	2.000
Rmeas	0.129	1.150
Number of reflections	57951	4118
<I/σ(I)>	8.7
Completeness [%]	97.8	93.8
Redundancy	3.8	3.5
CC(1/2)	0.996	0.462

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	0.1 M Tris-HCl at pH 7.4, 0.1 M MgCl2, 15% PEG 3350