6NOG

Poised-state Dot1L bound to the H2B-Ubiquitinated nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, E)	Histone H3.2	polymer	135	15303.9	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
2	C, D (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	E, F (C, G)	Histone H2A type 1	polymer	129	13978.2	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	G, H (D, H)	Histone H2B 1.1	polymer	122	13498.7	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I (L)	Ubiquitin	polymer	80	9036.4	1	UniProt (J3QS39) Pfam (PF00240)	Homo sapiens (Human)
6	J (K)	Histone-lysine N-methyltransferase, H3 lysine-79 specific	polymer	416	47432.0	1	UniProt (Q8TEK3) Pfam (PF08123)	Homo sapiens (Human)	DOT1-like protein, Histone H3-K79 methyltransferase, H3-K79-HMTase, Lysine N-methyltransferase 4, Dot1L
7	K (I)	601 DNA Strand 1	polymer	146	44825.6	1		synthetic construct
8	L (J)	601 DNA Strand 2	polymer	146	45305.9	1		synthetic construct

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation

C, G: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	engineered mutation
Ser 123	Ala 124	engineered mutation

D, H: 4 - 125 (UniProt: P02281)

PDB	External Database	Details
Thr 32	Ser 33	engineered mutation
Cys 120	Lys 121	engineered mutation

L: 1 - 76 (UniProt: J3QS39)

K: 2 - 416 (UniProt: Q8TEK3)

PDB	External Database	Details
Gly 1	-	expression tag

Sequence viewer

Contents of the asymmetric unit

*Water molecules are not included.