6NNJ
Crystal Structure of HIV-1 BG505 SOSIP.664 Prefusion Env Trimer Bound to CH31 scFv in Complex with Crystallization Chaperones 3H109L Fab and 35O22 scFv at 3.1 Angstrom
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | B | Envelope glycoprotein gp41 | polymer | 153 | 17146.5 | 1 | UniProt (Q2N0S6) Pfam (PF00517) In PDB | Human immunodeficiency virus 1 | |
| 2 | D | 35O22 scFv heavy chain | polymer | 153 | 15837.3 | 1 | Homo sapiens (human) | ||
| 3 | E | 35O22 scFv light chain | polymer | 130 | 14188.6 | 1 | Homo sapiens (human) | ||
| 4 | G | Envelope glycoprotein gp120 | polymer | 481 | 54021.3 | 1 | UniProt (Q2N0S6) Pfam (PF00516) In PDB | Human immunodeficiency virus 1 | |
| 5 | H | 3H109L Fab heavy chain | polymer | 244 | 26255.5 | 1 | Homo sapiens (human) | ||
| 6 | L | 3H109L Fab light chain | polymer | 217 | 23416.1 | 1 | Homo sapiens (human) | ||
| 7 | U | CH31 scFv heavy chain | polymer | 137 | 15340.1 | 1 | Homo sapiens (human) | ||
| 8 | V | CH31 scFv light chain | polymer | 117 | 11910.0 | 1 | Homo sapiens (human) | ||
| 9 | A | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G09724ZC) | |||
| 10 | C, J, K, M, O | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 5 | In PDB GlyTouCan (G42666HT) | |||
| 11 | F | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 12 | I | alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G22573RC) | |||
| 13 | N | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 1 | In PDB GlyTouCan (G40702WU) | |||
| 14 | B, G | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 7 | Chemie (NAG) |
Sequence modifications
B: 512 - 664 (UniProt: Q2N0S6)
G: 31 - 508 (UniProt: Q2N0S6)
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 556 | engineered mutation |
| Cys 605 | Thr 602 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 145 | Asn 136 | engineered mutation |
| Asn 332 | Thr 330 | engineered mutation |
| Cys 501 | Ala 498 | engineered mutation |
| Arg 509 | - | expression tag |
| Arg 510 | - | expression tag |
| Arg 511 | - | expression tag |
| Arg 512 | - | expression tag |
| Arg 513 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 178115.5 | |
| Branched | Number of molecules | 9 |
| Total formula weight | 6576.0 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1548.5 | |
| All* | Total formula weight | 186239.9 |
