6NNF
Crystal Structure of HIV-1 BG505 SOSIP.664 Prefusion Env Trimer Bound to VRC01 FR3-03 scFv in Complex with Crystallization Chaperones 3H109L Fab and 35O22 scFv at 3.5 Angstrom
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Envelope glycoprotein gp41 | polymer | 153 | 17146.5 | 1 | UniProt (Q2N0S6) Pfam (PF00517) | Human immunodeficiency virus 1 | |
| 2 | B (D) | 35O22 scFv heavy chain | polymer | 153 | 15837.3 | 1 | Homo sapiens (human) | ||
| 3 | C (E) | 35O22 scFv light chain | polymer | 130 | 14188.6 | 1 | Homo sapiens (human) | ||
| 4 | D (G) | Envelope glycoprotein gp120 | polymer | 481 | 54021.3 | 1 | UniProt (Q2N0S6) Pfam (PF00516) | Human immunodeficiency virus 1 | |
| 5 | E (H) | 3H109L Fab heavy chain | polymer | 244 | 26255.5 | 1 | Homo sapiens (human) | ||
| 6 | F (L) | 3H109L Fab light chain | polymer | 217 | 23416.1 | 1 | Homo sapiens (human) | ||
| 7 | G (U) | VRC01 FR3-03 heavy chain | polymer | 142 | 16232.3 | 1 | Homo sapiens (human) | ||
| 8 | H (V) | VRC01 FR3-03 light chain | polymer | 115 | 11931.0 | 1 | Homo sapiens (human) | ||
| 9 | I (A) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G09724ZC) | |||
| 10 | J, L, N, O, P... (C, I, K, M, N...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 7 | In PDB GlyTouCan (G42666HT) | |||
| 11 | K (F) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 12 | M (J) | alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G22573RC) | |||
| 13 | Q (O) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1721.5 | 1 | In PDB GlyTouCan (G40702WU) | |||
| 14 | T, U, V, W, X (B, G) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) |
Sequence modifications
B: 512 - 664 (UniProt: Q2N0S6)
G: 31 - 508 (UniProt: Q2N0S6)
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 556 | engineered mutation |
| Cys 605 | Thr 602 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 137 | Asn 136 | engineered mutation |
| Asn 332 | Thr 330 | engineered mutation |
| Cys 501 | Ala 498 | engineered mutation |
| Arg 509 | - | expression tag |
| Arg 510 | - | expression tag |
| Arg 511 | - | expression tag |
| Arg 512 | - | expression tag |
| Arg 513 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 179028.7 | |
| Branched | Number of molecules | 11 |
| Total formula weight | 7424.8 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1106.0 | |
| All* | Total formula weight | 187559.5 |
