6NNF
Crystal Structure of HIV-1 BG505 SOSIP.664 Prefusion Env Trimer Bound to VRC01 FR3-03 scFv in Complex with Crystallization Chaperones 3H109L Fab and 35O22 scFv at 3.5 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-04 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | P 63 |
Unit cell lengths | 130.870, 130.870, 315.190 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.810 |
High resolution limit [Å] | 2.760 | 7.210 | 2.760 |
Rmerge | 0.053 | 0.026 | 0.882 |
Rmeas | 0.067 | 0.033 | 1.158 |
Rpim | 0.041 | 0.019 | 0.739 |
Total number of observations | 78976 | ||
Number of reflections | 36136 | 4089 | 43 |
<I/σ(I)> | 6.7 | ||
Completeness [%] | 40.7 | 90.9 | 1 |
Redundancy | 2.2 | 2.2 | 1.8 |
CC(1/2) | 0.989 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 298 | 72 mM imidazole pH 6.5, 72 mM |