6NNF

Crystal Structure of HIV-1 BG505 SOSIP.664 Prefusion Env Trimer Bound to VRC01 FR3-03 scFv in Complex with Crystallization Chaperones 3H109L Fab and 35O22 scFv at 3.5 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-04
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	130.870, 130.870, 315.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.837 - 2.762
R-factor	0.2445
Rwork	0.242
R-free	0.29760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tvp 4lst
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.810
High resolution limit [Å]	2.760	7.210	2.760
Rmerge	0.053	0.026	0.882
Rmeas	0.067	0.033	1.158
Rpim	0.041	0.019	0.739
Total number of observations	78976
Number of reflections	36136	4089	43
<I/σ(I)>	6.7
Completeness [%]	40.7	90.9	1
Redundancy	2.2	2.2	1.8
CC(1/2)		0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	72 mM imidazole pH 6.5, 72 mM