6NN6

Structure of Dot1L-H2BK120ub nucleosome complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15303.9	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A type 1	polymer	129	13978.2	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B 1.1	polymer	124	13642.8	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I (I)	DNA (145-MER)	polymer	145	44520.4	1		synthetic construct
6	J (J)	DNA (145-MER)	polymer	145	44991.7	1		synthetic construct
7	K (K)	Histone-lysine N-methyltransferase, H3 lysine-79 specific	polymer	416	47462.0	1	UniProt (Q8TEK3) Pfam (PF08123)	Homo sapiens (Human)	DOT1-like protein,Histone H3-K79 methyltransferase,H3-K79-HMTase,Lysine N-methyltransferase 4
8	L (L)	Ubiquitin	polymer	80	8911.2	1	UniProt (F5H388) Pfam (PF00240)	Homo sapiens (Human)

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation

C, G: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	engineered mutation
Ser 123	Ala 124	engineered mutation

D, H: 4 - 125 (UniProt: P02281)

PDB	External Database	Details
Gly 2	-	expression tag
Ser 3	-	expression tag
Thr 32	Ser 33	engineered mutation
Cys 120	Lys 121	engineered mutation

K: 3 - 416 (UniProt: Q8TEK3)

PDB	External Database	Details
Gly 1	-	expression tag
Ser 2	-	expression tag

L: -1 - 75 (UniProt: F5H388)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser 0	Gly 76	engineered mutation
Cys 76	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	254261.8
All*	Total formula weight	254261.8

*Water molecules are not included.