6NM9
CryoEM structure of the LbCas12a-crRNA-AcrVA4 dimer
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, C) | AcrVA4 | polymer | 234 | 27369.2 | 2 | UniProt (A0A0U2APF4) | Moraxella bovoculi | |
| 2 | B, E (B, D) | Cpf1 | polymer | 1227 | 143750.2 | 2 | UniProt (A0A182DWE3) | Lachnospiraceae bacterium ND2006 | |
| 3 | C, F (G, E) | RNA (25-MER) | polymer | 40 | 12879.6 | 2 | Lachnospiraceae bacterium ND2006 | ||
| 4 | G, H, I, J (A, B, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) |
Sequence modifications
B, D: 2 - 1227 (UniProt: A0A182DWE3)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| Asn 112 | Ala 113 | conflict |
| Glu 113 | Ala 114 | conflict |
| Phe 131 | Ala 132 | conflict |
| Leu 132 | Ala 133 | conflict |
| Gln 264 | Ala 265 | conflict |
| Lys 269 | Ala 270 | conflict |
| Val 357 | Leu 358 | conflict |
| Arg 1076 | Ala 1077 | conflict |
| Asn 1077 | Ala 1078 | conflict |
| Pro 1078 | Ala 1079 | conflict |
| Asp 1085 | Ala 1086 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 367998.0 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 97.2 | |
| All* | Total formula weight | 368095.3 |
