6NM9
CryoEM structure of the LbCas12a-crRNA-AcrVA4 dimer
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.38 |
| Software | cisTEM |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING ONLY |
