6NIL

cryoEM structure of the truncated HIV-1 Vif/CBFbeta/A3F complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J	DNA dC->dU-editing enzyme APOBEC-3F	polymer	207	24433.3	4	UniProt (Q8IUX4) Pfam (PF08210) In PDB	Homo sapiens (Human)	Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F,A3F
2	B, E, H, K	Core-binding factor subunit beta	polymer	151	17851.0	4	UniProt (Q13951) Pfam (PF02312) In PDB	Homo sapiens (Human)	CBF-beta,Polyomavirus enhancer-binding protein 2 beta subunit,PEBP2-beta,SL3-3 enhancer factor 1 subunit beta,SL3/AKV core-binding factor beta subunit
3	C, F, I, L	Virion infectivity factor	polymer	138	16736.1	4	UniProt (P12504) UniProt (A0A346ARH7) Pfam (PF00559) In PDB	Human immunodeficiency virus 1 (HIV-1)	Vif,SOR protein
4	A, D, G, J	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A, D, G, J: 185 - 373 (UniProt: Q8IUX4)

PDB	External Database	Details
Met 167	-	initiating methionine
Gly 168	-	expression tag
Ser 169	-	expression tag
Ser 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag
His 175	-	expression tag
His 176	-	expression tag
Ser 177	-	expression tag
Gln 178	-	expression tag
Asp 179	-	expression tag
Pro 180	-	expression tag
Asn 181	-	expression tag
Ser 182	-	expression tag
Met 183	-	expression tag
Gly 184	-	expression tag
Asp 196	Tyr 196	engineered mutation
Gly 247	His 247	engineered mutation
Arg 248	Cys 248	engineered mutation
Lys 302	Phe 302	engineered mutation
Lys 310	Trp 310	engineered mutation
Ala 314	Tyr 314	engineered mutation
Ala 315	Gln 315	engineered mutation
Asp 355	Lys 355	engineered mutation
Asp 358	Lys 358	engineered mutation
Asp 363	Phe 363	engineered mutation

C, F, I, L: 1 - 113 (UniProt: P12504)

PDB	External Database	Details
Glu 114	-	linker
Ala 153	-	linker
Ser 154	-	linker
Glu 155	-	linker
Gly 156	-	linker
Ser 157	-	linker

C, F, I, L: 158 - 176 (UniProt: A0A346ARH7)

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	236081.7
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	236343.3

*Water molecules are not included.