6NI3

B2V2R-Gs protein subcomplex of a GPCR-G protein-beta-arrestin mega-complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	380	44326.2	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (Human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (R)	Endolysin,Beta-2 adrenergic receptor chimera	polymer	506	57630.8	1	UniProt (A0A097J809) UniProt (P07550) Pfam (PF00001)	Enterobacteria phage RB59	Lysis protein,Lysozyme,Muramidase,Beta-2 adrenoreceptor,Beta-2 adrenoceptor
5	E (N)	Nanobody 35	polymer	138	15140.7	1		Lama glama (Llama)
6	F (R)	8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one	non-polymer		370.4	1	Chemie (P0G)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
-	Glu 71	deletion
-	Gly 72	deletion
-	Gly 73	deletion
-	Glu 74	deletion
-	Glu 75	deletion
-	Asp 76	deletion
-	Pro 77	deletion
-	Gln 78	deletion
-	Ala 79	deletion
-	Ala 80	deletion
-	Arg 81	deletion
-	Ser 82	deletion
-	Asn 83	deletion
-	Ser 84	deletion
Ser 86	Gly 86	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: -133 - 26 (UniProt: A0A097J809)

PDB	External Database	Details
Met -164	-	expression tag
Lys -163	-	expression tag
Thr -162	-	expression tag
Ile -161	-	expression tag
Ile -160	-	expression tag
Ala -159	-	expression tag
Leu -158	-	expression tag
Ser -157	-	expression tag
Tyr -156	-	expression tag
Ile -155	-	expression tag
Phe -154	-	expression tag
Cys -153	-	expression tag
Leu -152	-	expression tag
Val -151	-	expression tag
Phe -150	-	expression tag
Ala -149	-	expression tag
Asp -148	-	expression tag
Tyr -147	-	expression tag
Lys -146	-	expression tag
Asp -145	-	expression tag
Asp -144	-	expression tag
Asp -143	-	expression tag
Asp -142	-	expression tag
Ala -141	-	expression tag
Glu -140	-	expression tag
Asn -139	-	expression tag
Leu -138	-	expression tag
Tyr -137	-	expression tag
Phe -136	-	expression tag
Gln -135	-	expression tag
Gly -134	-	expression tag
Thr -81	Cys 54	conflict
Ala -38	Cys 97	conflict
Ala 27	-	linker
Ala 28	-	linker

R: 29 - 341 (UniProt: P07550)

PDB	External Database	Details
Thr 96	Met 96	conflict
Thr 98	Met 98	conflict
Glu 187	Asn 187	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	163492.9
Non-Polymers*	Number of molecules	1
	Total formula weight	370.4
All*	Total formula weight	163863.3

*Water molecules are not included.