6NHR

Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin HA2 I45F mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E	Hemagglutinin HA1 chain	polymer	321	35320.8	3	UniProt (H9XC94) Pfam (PF00509) In PDB	Influenza A virus (strain A/Hong Kong/1/1968 H3N2)
2	B, D, F	Hemagglutinin HA2 chain	polymer	176	20231.3	3	UniProt (Q91MA7) Pfam (PF00509) In PDB	Influenza A virus (strain A/Hong Kong/1/1968 H3N2)
3	G, H	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
4	I, K	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
5	J	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G22573RC)
6	L	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
7	A, C, D, E	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	7	Chemie (NAG)
8		water	water		18.0	846	Chemie (HOH)

Sequence modifications

A, C, E: 11 - 329 (UniProt: H9XC94)

PDB	External Database	Details
Pro 9	-	expression tag
Gly 10	-	expression tag
Ser 145	Unk 161	variant
Leu 226	Met 242	conflict

B, D, F: 1 - 176 (UniProt: Q91MA7)

PDB	External Database	Details
Phe 45	Ile 390	engineered mutation
Gly 123	Arg 468	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	166656.3
Branched	Number of molecules	6
	Total formula weight	3519.3
Non-Polymers*	Number of molecules	7
	Total formula weight	1548.5
All*	Total formula weight	171724.0

*Water molecules are not included.