6NHR
Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin HA2 I45F mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 208.378, 131.409, 72.280 |
Unit cell angles | 90.00, 98.07, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.18588 |
Rwork | 0.184 |
R-free | 0.21614 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fnk |
RMSD bond length | 0.011 |
RMSD bond angle | 1.497 |
Data scaling software | HKL-2000 (712) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.050 | 0.400 |
Number of reflections | 110282 | 13871 |
<I/σ(I)> | 20 | 1.7 |
Completeness [%] | 97.4 | 98.1 |
Redundancy | 5.6 | 5.2 |
CC(1/2) | 1.000 | 0.770 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M sodium cacodylate pH 6.5, 5% PEG 8000, and 38% 2-methyl-2,4-pentanediol |