6NCS

Crystal structure of N-acetylneuraminic acid (Sialic acid) synthetase from Leptospira borgpetersenii serovar Hardjo-bovis in complex with citrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	N-acetylneuraminic acid (Sialic acid) synthetase	polymer	290	32286.1	2	UniProt (Q04TM5) Pfam (PF03102)	Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
2	C, R (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	D, S (A, B)	CITRIC ACID	non-polymer		192.1	2	Chemie (CIT)
4	E (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
5	F (A)	FORMIC ACID	non-polymer		46.0	1	Chemie (FMT)
6	G (A)	D(-)-TARTARIC ACID	non-polymer		150.1	1	Chemie (TAR)
7	AA, BA, CA, H, I... (B, A)	IODIDE ION	non-polymer		126.9	20	Chemie (IOD)
8	DA, EA (A, B)	water	water		18.0	518	Chemie (HOH)

Sequence modifications

A, B: 1 - 282 (UniProt: Q04TM5)

PDB	External Database	Details
Met -7	-	initiating methionine
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	64572.2
Non-Polymers*	Number of molecules	27
	Total formula weight	3287.4
All*	Total formula weight	67859.5

*Water molecules are not included.