6NBH

Cryo-EM structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and G protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Parathyroid hormone/parathyroid hormone-related peptide receptor	polymer	478	54755.0	1	UniProt (Q03431) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (Human)	PTH/PTHrP type I receptor,PTH/PTHr receptor,Parathyroid hormone 1 receptor,PTH1 receptor
2	P	Long-acting parathyroid hormone analog	polymer	36	4274.0	1		Homo sapiens
3	A	Gs protein alpha subunit	polymer	378	43867.8	1	Pfam (PF00503) UniProt (by SIFTS) (P63097) UniProt (by SIFTS) (P04896) In PDB	Bos taurus
4	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Rat)	Transducin beta chain 1
5	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (Bovine)	G gamma-I
6	N	Nanobody-35	polymer	126	13711.3	1		synthetic construct
7	R	CHOLESTEROL	non-polymer		386.7	7	Chemie (CLR)
8	R	PALMITIC ACID	non-polymer		256.4	3	Chemie (PLM)

Sequence modifications

R: 27 - 502 (UniProt: Q03431)

PDB	External Database	Details
Ala 188	Gly 188	engineered mutation
Glu 503	-	expression tag
Phe 504	-	expression tag

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	162384.7
Non-Polymers*	Number of molecules	10
	Total formula weight	3475.8
All*	Total formula weight	165860.6

*Water molecules are not included.