6N85
Resistance to inhibitors of cholinesterase 8A (Ric8A) protein in complex with MBP-tagged transducin-alpha residues 327-350
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | B | Synembryn-A | polymer | 512 | 57465.0 | 1 | UniProt (Q5E9J8) Pfam (PF10165) In PDB | Bos taurus (Bovine) | Protein Ric-8A |
2 | M | Maltose/maltodextrin-binding periplasmic protein,Guanine nucleotide-binding protein G(t) subunit alpha-2 | polymer | 415 | 45448.4 | 1 | UniProt (P0AEY0) UniProt (P19087) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) In PDB | Escherichia coli O157:H7 | MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Transducin alpha-2 chain |
3 | A | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
4 | water | water | 18.0 | 95 | Chemie (HOH) |
Sequence modifications
B: 1 - 492 (UniProt: Q5E9J8)
M: 2 - 367 (UniProt: P0AEY0)
M: 372 - 395 (UniProt: P19087)
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Ala 460 | Glu 460 | engineered mutation |
Ala 461 | Glu 461 | engineered mutation |
Ala 462 | Lys 462 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Met 1 | - | expression tag |
Ala 83 | Asp 108 | engineered mutation |
Ala 84 | Lys 109 | engineered mutation |
Ala 173 | Glu 198 | engineered mutation |
Ala 174 | Asn 199 | engineered mutation |
His 216 | Ala 241 | engineered mutation |
His 220 | Lys 245 | engineered mutation |
Ala 240 | Lys 265 | engineered mutation |
Ala 368 | - | linker |
Ala 369 | - | linker |
Ala 370 | - | linker |
His 371 | - | linker |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 102913.4 | |
Branched | Number of molecules | 1 |
Total formula weight | 342.3 | |
All* | Total formula weight | 103255.7 |