6N85

Resistance to inhibitors of cholinesterase 8A (Ric8A) protein in complex with MBP-tagged transducin-alpha residues 327-350

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-02-07
Detector	RDI CMOS_8M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	65.740, 91.920, 86.230
Unit cell angles	90.00, 112.30, 90.00

Refinement procedure

Resolution	41.386 - 2.500
R-factor	0.1991
Rwork	0.196
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.478
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.180	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.066	0.716
Rmeas	0.080	0.871
Rpim	0.030	0.332
Number of reflections	32806	4784
<I/σ(I)>	20.4	2.6
Completeness [%]	99.8	99.8
Redundancy	7.1	6.7
CC(1/2)	0.999	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.1 M MIB buffer, 25 % PEG3350pH 8.0