6N84

MBP-fusion protein of transducin-alpha residues 327-350

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Maltose/maltodextrin-binding periplasmic protein,Guanine nucleotide-binding protein G(t) subunit alpha-2	polymer	415	45448.4	1	UniProt (P0AEY0) UniProt (P19087) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) In PDB	Escherichia coli O157:H7	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Transducin alpha-2 chain
2	B	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	A	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
4		water	water		18.0	478	Chemie (HOH)

Sequence modifications

A: 2 - 367 (UniProt: P0AEY0)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Met 1	-	expression tag
Ala 83	Asp 108	engineered mutation
Ala 84	Lys 109	engineered mutation
Ala 173	Glu 198	engineered mutation
Ala 174	Asn 199	engineered mutation
His 216	Ala 241	engineered mutation
His 220	Lys 245	engineered mutation
Ala 240	Lys 265	engineered mutation
Ala 368	-	linker
Ala 369	-	linker
Ala 370	-	linker
His 371	-	linker

A: 372 - 395 (UniProt: P19087)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45448.4
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	8
	Total formula weight	768.5
All*	Total formula weight	46559.2

*Water molecules are not included.