6N84

MBP-fusion protein of transducin-alpha residues 327-350

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-10-10
Detector	RDI CMOS_8M
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	83.070, 83.070, 109.229
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.080 - 1.750
R-factor	0.1579
Rwork	0.157
R-free	0.18490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf
RMSD bond length	0.006
RMSD bond angle	0.794
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.080	1.840
High resolution limit [Å]	1.750	1.750
Rmerge	0.062	0.753
Rmeas	0.069	0.849
Rpim	0.021	0.277
Number of reflections	43096	6311
<I/σ(I)>	29	2.5
Completeness [%]	100.0	100
Redundancy	10.7	9.3
CC(1/2)	1.000	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	2 M Ammonium Sulphate, 0.1 M sodium acetate, pH 4.6