6N4R

CryoEM structure of Nav1.7 VSD2 (deactived state) in complex with the gating modifier toxin ProTx2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, L (B, C, D, A)	Nav1.7 VSD2-NavAb chimera	polymer	288	33453.5	4	UniProt (A8EVM5) UniProt (Q15858) Pfam (PF00520) Pfam (PF08016)	Arcobacter butzleri (strain RM4018)	Neuroendocrine sodium channel,hNE-Na,Peripheral sodium channel 1,PN1,Sodium channel protein type IX subunit alpha,Voltage-gated sodium channel subunit alpha Nav1.7
2	D, E, F, G (E, F, G, H)	Beta/omega-theraphotoxin-Tp2a	polymer	30	3839.7	4	UniProt (P83476)	Thrixopelma pruriens	Beta/omega-TRTX-Tp2a,ProTx-II,PT-II,Protoxin-2,ProTx2
3	H, J (I, K)	Fab light chain	polymer	215	23483.9	2		Mus musculus (mouse)
4	I, K (J, L)	Fab heavy chain	polymer	228	24523.5	2		Mus musculus (mouse)

Sequence modifications

B, C, D, A: 722 - 746 (UniProt: A8EVM5)

PDB	External Database	Details
Met 704	-	initiating methionine
Asp 705	-	expression tag
Tyr 706	-	expression tag
Lys 707	-	expression tag
Asp 708	-	expression tag
Asp 709	-	expression tag
Asp 710	-	expression tag
Asp 711	-	expression tag
Lys 712	-	expression tag
Gly 713	-	expression tag
Ser 714	-	expression tag
Leu 715	-	expression tag
Val 716	-	expression tag
Pro 717	-	expression tag
Arg 718	-	expression tag
Gly 719	-	expression tag
Ser 720	-	expression tag
His 721	-	expression tag

B, C, D, A: 747 - 777 (UniProt: Q15858)
B, C, D, A: 778 - 798 (UniProt: A8EVM5)
B, C, D, A: 799 - 830 (UniProt: Q15858)
B, C, D, A: 831 - 991 (UniProt: A8EVM5)

PDB	External Database	Details
Cys 941	Ile 217	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	245187.7
All*	Total formula weight	245187.7

*Water molecules are not included.