6N4C
EM structure of the DNA wrapping in bacterial open transcription initiation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (F) | RNA polymerase sigma factor RpoD | polymer | 558 | 64322.3 | 1 | UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Escherichia coli K-12 | Sigma-70 |
| 2 | B (C) | DNA-directed RNA polymerase subunit beta | polymer | 1341 | 150689.7 | 1 | UniProt (A0A0A0GWV9) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) UniProt (by SIFTS) (P0A8V2) | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | C (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1358 | 150320.2 | 1 | UniProt (A0A369F490) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | D (A) | DNA-directed RNA polymerase subunit alpha | polymer | 316 | 35275.3 | 1 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 5 | E (B) | DNA-directed RNA polymerase subunit alpha | polymer | 310 | 34400.3 | 1 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 6 | F (E) | DNA-directed RNA polymerase subunit omega | polymer | 90 | 10118.4 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 7 | G (a) | DNA (94-MER) | polymer | 94 | 29094.6 | 1 | Enterobacteria phage lambda | ||
| 8 | H (b) | DNA (94-MER) | polymer | 94 | 28890.5 | 1 | Enterobacteria phage lambda |
Sequence modifications
F: 8 - 613 (UniProt: P00579)
D: 15 - 1376 (UniProt: A0A369F490)
B: 6 - 315 (UniProt: P0A7Z4)
| PDB | External Database | Details |
|---|---|---|
| - | Glu 172 | deletion |
| - | Asp 173 | deletion |
| - | Leu 174 | deletion |
| - | Ala 175 | deletion |
| - | Pro 176 | deletion |
| - | Thr 177 | deletion |
| - | Ala 178 | deletion |
| - | Thr 179 | deletion |
| - | His 180 | deletion |
| - | Val 181 | deletion |
| - | Gly 182 | deletion |
| - | Ser 183 | deletion |
| - | Glu 184 | deletion |
| - | Leu 185 | deletion |
| - | Ser 186 | deletion |
| - | Gln 187 | deletion |
| - | Glu 188 | deletion |
| - | Asp 189 | deletion |
| - | Leu 190 | deletion |
| - | Asp 191 | deletion |
| - | Asp 192 | deletion |
| - | Asp 193 | deletion |
| - | Glu 194 | deletion |
| - | Asp 195 | deletion |
| - | Glu 196 | deletion |
| - | Asp 197 | deletion |
| - | Glu 198 | deletion |
| - | Glu 199 | deletion |
| - | Asp 200 | deletion |
| - | Gly 201 | deletion |
| - | Asp 202 | deletion |
| - | Asp 203 | deletion |
| - | Asp 204 | deletion |
| - | Ser 205 | deletion |
| - | Ala 206 | deletion |
| - | Asp 207 | deletion |
| - | Asp 208 | deletion |
| - | Asp 209 | deletion |
| - | Ile 500 | deletion |
| - | Ala 501 | deletion |
| - | Lys 502 | deletion |
| - | Glu 503 | deletion |
| - | Pro 504 | deletion |
| Ile 521 | - | insertion |
| - | Leu 528 | deletion |
| - | Glu 529 | deletion |
| - | Leu 530 | deletion |
| - | Pro 531 | deletion |
| - | Leu 532 | deletion |
| - | Asp 533 | deletion |
| PDB | External Database | Details |
|---|---|---|
| - | Glu 523 | deletion |
| - | Gly 524 | deletion |
| - | Lys 531 | deletion |
| - | Glu 532 | deletion |
| PDB | External Database | Details |
|---|---|---|
| Leu 255 | Arg 255 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 503111.2 | |
| All* | Total formula weight | 503111.2 |
