6N4C
EM structure of the DNA wrapping in bacterial open transcription initiation complex
EM specimen
| Staining | Yes |
| Staining material | Uranyl Acetate |
EM Data Collection
| Microscope | JEOL 2100 |
| Electron Gun | LAB6 |
| Accelerating Voltage (kV) | 200 |
| Detector | TVIPS TEMCAM-F416 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 17 |
| Number of Particle | 60393 |
| Software | IMAGIC,UCSF Chimera |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
