6MZ3

mCherry pH sensitive mutant - M66T (mCherryTYG)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	PAmCherry1 protein	polymer	270	30560.3	1	UniProt (D1MPT3) Pfam (PF01353) In PDB	Discosoma sp. (Sea anemone)
2		water	water		18.0	313	Chemie (HOH)

Sequence modifications

A: -4 - 231 (UniProt: D1MPT3)

PDB	External Database	Details
Met -38	-	initiating methionine
Arg -37	-	expression tag
Gly -36	-	expression tag
Ser -35	-	expression tag
His -34	-	expression tag
His -33	-	expression tag
His -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
Gly -28	-	expression tag
Met -27	-	expression tag
Ala -26	-	expression tag
Ser -25	-	expression tag
Met -24	-	expression tag
Thr -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Gln -20	-	expression tag
Gln -19	-	expression tag
Met -18	-	expression tag
Gly -17	-	expression tag
Arg -16	-	expression tag
Asp -15	-	expression tag
Leu -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Asp -9	-	expression tag
Lys -8	-	expression tag
Asp -7	-	expression tag
Pro -6	-	expression tag
Thr -5	-	expression tag
Glu 26	Val 31	conflict
Ala 57	Thr 62	conflict
Cro 66	Met 71	chromophore
-	Tyr 72	chromophore
-	Gly 73	chromophore
Lys 70	Asn 75	conflict
Leu 83	Phe 88	conflict
Asn 98	Lys 103	conflict
Ser 146	Leu 151	conflict
Ile 161	Val 166	conflict
Gln 163	Pro 168	conflict
Leu 165	Val 170	conflict
Ile 197	Arg 202	conflict

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30560.3
All*	Total formula weight	30560.3

*Water molecules are not included.