6MZ3
mCherry pH sensitive mutant - M66T (mCherryTYG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.088 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.806, 42.782, 61.067 |
Unit cell angles | 90.00, 112.20, 90.00 |
Refinement procedure
Resolution | 19.979 - 1.088 |
R-factor | 0.1671 |
Rwork | 0.167 |
R-free | 0.17760 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.992 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.979 | 1.210 |
High resolution limit [Å] | 1.088 | 1.090 |
Number of reflections | 76260 | |
<I/σ(I)> | 10.4 | |
Completeness [%] | 93.6 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 50 mM Tris-HCl, 100 mM NaOAc, 30% PEG 4000, pH 8.5 |