6MM8

Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 K2879A, S2813D phosphomimetic (2699-2904) crystal form 2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	cAMP-dependent protein kinase catalytic subunit alpha	polymer	339	39598.2	1	UniProt (P05132) Pfam (PF00069) In PDB	Mus musculus (Mouse)	PKA C-alpha
2	D	Ryanodine receptor 2	polymer	209	24285.6	1	UniProt (E9Q401) Pfam (PF02026) In PDB	Mus musculus (Mouse)	RyR2,Cardiac muscle ryanodine receptor,Cardiac muscle ryanodine receptor-calcium release channel,Type 2 ryanodine receptor
3	C	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
4	C, D	ACETIC ACID	non-polymer		60.1	2	Chemie (ACY)
5	C	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
6	C, D	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
7	D	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
8		water	water		18.0	503	Chemie (HOH)

Sequence modifications

C: 15 - 350 (UniProt: P05132)

PDB	External Database	Details
Ser 12	-	expression tag
Asn 13	-	expression tag
Ala 14	-	expression tag

D: 2699 - 2904 (UniProt: E9Q401)

PDB	External Database	Details
Ser 2696	-	expression tag
Asn 2697	-	expression tag
Ala 2698	-	expression tag
Asp 2813	Ser 2813	engineered mutation
Ala 2879	Lys 2879	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	63883.8
Non-Polymers*	Number of molecules	10
	Total formula weight	1078.2
All*	Total formula weight	64962.0

*Water molecules are not included.