6MGL

Crystal structure of the catalytic domain from GH74 enzyme PoGH74 from Paenibacillus odorifer, D60A mutant in complex with XXLG and XGXXLG xyloglucan

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Xyloglucanase	polymer	747	80139.3	1	UniProt (A0A1R0YRH0) In PDB	Paenibacillus odorifer
2	B	alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[beta-D-galactopyranose-(1-2)-alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		1225.1	1	In PDB GlyTouCan (G18071YH)
3	C	alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[beta-D-galactopyranose-(1-2)-alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		1681.5	1	In PDB GlyTouCan (G42956CV)
4	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	A	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
6	A	3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL	non-polymer		634.8	1	Chemie (PE3)
7	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
8		water	water		18.0	1399	Chemie (HOH)

Sequence modifications

A: 1 - 745 (UniProt: A0A1R0YRH0)

PDB	External Database	Details
Gly -1	-	expression tag
Met 0	-	expression tag
Ala 36	Asp 70	engineered mutation
Gly 600	Ala 634	variant
Ser 680	Ala 714	variant

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	80139.3
Branched	Number of molecules	2
	Total formula weight	2906.5
Non-Polymers*	Number of molecules	9
	Total formula weight	1054.9
All*	Total formula weight	84100.6

*Water molecules are not included.