6MFR

Human Argonaute2-miR-122 bound to a target RNA with three central mismatches (bu3)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Protein argonaute-2	polymer	859	97358.2	2	UniProt (Q9UKV8) Pfam (PF16486) Pfam (PF08699) Pfam (PF02170) Pfam (PF16488) Pfam (PF16487) Pfam (PF02171)	Homo sapiens (Human)	hAgo2,Argonaute RISC catalytic component 2,Eukaryotic translation initiation factor 2C 2,eIF2C 2,PAZ Piwi domain protein,PPD,Protein slicer
2	C, D (C, D)	RNA (5'-R(P*UP*GP*GP*AP*GP*UP*GP*UP*GP*AP*CP*AP*AP*UP*GP*GP*UP*GP*UP*U)-3')	polymer	21	6788.0	2		Homo sapiens
3	E, F (E, F)	RNA (5'-R(P*AP*AP*AP*CP*AP*CP*CP*AP*UP*UP*UP*UP*CP*AP*CP*AP*CP*UP*CP*CP*AP*AP*A)-3')	polymer	23	7242.4	2		unidentified
4	G, H, J, K (A, B)	PHENOL	non-polymer		94.1	4	Chemie (IPH)
5	I (B)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)

Sequence modifications

A, B: 1 - 859 (UniProt: Q9UKV8)

PDB	External Database	Details
Asp 387	Ser 387	engineered mutation
Ala 669	Asp 669	engineered mutation
Ala 824	Ser 824	engineered mutation
Asp 828	Ser 828	engineered mutation
Asp 831	Ser 831	engineered mutation
Ala 834	Ser 834	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	222777.3
Non-Polymers*	Number of molecules	5
	Total formula weight	471.4
All*	Total formula weight	223248.7

*Water molecules are not included.