6MDR

Cryo-EM structure of the Ceru+32/GFP-17 protomer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	a, c, e, g, i...	Ceru+32	polymer	247	28425.2	8	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria (Jellyfish)	Green fluorescent protein
2	b, d, f, h, j...	GFP-17	polymer	240	27226.2	8	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria (Jellyfish)	Green fluorescent protein

Sequence modifications

a, c, e, g, i, k, m, o: 4 - 233 (UniProt: P42212)

PDB	External Database	Details
Met 1	-	expression tag
Ala 2	-	expression tag
Ser 3	-	expression tag
Arg 7	Glu 6	engineered mutation
Arg 10	Thr 9	engineered mutation
Lys 12	Val 11	engineered mutation
Lys 20	Asp 19	engineered mutation
Arg 31	Ser 30	engineered mutation
Lys 33	Glu 32	engineered mutation
Lys 35	Glu 34	engineered mutation
Asn 40	Tyr 39	engineered mutation
Leu 65	Phe 64	engineered mutation
Thr 66	Ser 65	engineered mutation
Trp 67	Tyr 66	engineered mutation
Ala 73	Ser 72	engineered mutation
Lys 77	Asp 76	engineered mutation
Arg 81	Gln 80	engineered mutation
Lys 91	Glu 90	engineered mutation
Ser 100	Phe 99	engineered mutation
Lys 103	Asp 102	engineered mutation
Thr 106	Asn 105	engineered mutation
Arg 118	Asp 117	engineered mutation
Lys 125	Glu 124	engineered mutation
Arg 129	Ile 128	engineered mutation
Lys 134	Asp 133	engineered mutation
Arg 143	Glu 142	engineered mutation
Gly 146	Tyr 145	engineered mutation
Ile 147	Asn 146	engineered mutation
Asp 149	His 148	engineered mutation
Lys 150	Asn 149	engineered mutation
Thr 154	Met 153	engineered mutation
Arg 158	Gln 157	engineered mutation
Ala 164	Val 163	engineered mutation
Lys 165	Asn 164	engineered mutation
Val 172	Ile 171	engineered mutation
Lys 173	Glu 172	engineered mutation
Arg 191	Asp 190	engineered mutation
Arg 198	Asp 197	engineered mutation
Arg 205	Gln 204	engineered mutation
Val 207	Ala 206	engineered mutation
Lys 213	Asn 212	engineered mutation
Lys 231	Thr 230	engineered mutation
Arg 234	-	expression tag
Asp 235	-	expression tag
Glu 236	-	expression tag
Arg 237	-	expression tag
Tyr 238	-	expression tag
Lys 239	-	expression tag
Leu 240	-	expression tag
Glu 241	-	expression tag
His 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag
His 245	-	expression tag
His 246	-	expression tag
His 247	-	expression tag

b, d, f, h, j, l, n, p: 4 - 234 (UniProt: P42212)

PDB	External Database	Details
Met 3	-	initiating methionine
Arg 32	Ser 30	engineered mutation
Asp 40	Thr 38	engineered mutation
Asn 41	Tyr 39	engineered mutation
Asp 45	Thr 43	engineered mutation
Leu 66	Phe 64	engineered mutation
Thr 67	Ser 65	engineered mutation
Glu 82	Gln 80	engineered mutation
Ser 101	Phe 99	engineered mutation
Thr 107	Asn 105	engineered mutation
Phe 147	Tyr 145	engineered mutation
Glu 151	Asn 149	engineered mutation
Thr 155	Met 153	engineered mutation
Asp 158	Lys 156	engineered mutation
Glu 159	Gln 157	engineered mutation
Ala 165	Val 163	engineered mutation
Glu 166	Asn 164	engineered mutation
Val 173	Ile 171	engineered mutation
Asp 195	Val 193	engineered mutation
Glu 200	Asn 198	engineered mutation
Val 208	Ala 206	engineered mutation
Asp 229	Ala 227	engineered mutation
Glu 233	His 231	engineered mutation
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag
His 238	-	expression tag
His 239	-	expression tag
His 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	445211.5
All*	Total formula weight	445211.5

*Water molecules are not included.