6M9T
Crystal structure of EP3 receptor bound to misoprostol-FA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Prostaglandin E2 receptor EP3 subtype, Endolysin chimera | polymer | 537 | 60023.4 | 1 | UniProt (P43115) UniProt (P00720) Pfam (PF00001) Pfam (PF00959) | Homo sapiens (Human) | PGE2 receptor EP3 subtype,PGE2-R,Prostanoid EP3 receptor,Lysis protein,Lysozyme,Muramidase |
| 2 | B (A) | (11alpha,12alpha,13E,16S)-11,16-dihydroxy-16-methyl-9-oxoprost-13-en-1-oic acid | non-polymer | 368.5 | 1 | Chemie (J9P) | |||
| 3 | C, D, E, F (A) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 4 | G (A) | (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate | non-polymer | 356.5 | 1 | Chemie (OLC) | |||
| 5 | H, I, J, K (A) | OLEIC ACID | non-polymer | 282.5 | 4 | Chemie (OLA) | |||
| 6 | L (A) | water | water | 18.0 | 5 | Chemie (HOH) |
Sequence modifications
A: 2 - 259 (UniProt: P43115)
A: 1002 - 1161 (UniProt: P00720)
A: 273 - 353 (UniProt: P43115)
| PDB | External Database | Details |
|---|---|---|
| Val -11 | - | expression tag |
| Phe -10 | - | expression tag |
| Ala -9 | - | expression tag |
| Asp -8 | - | expression tag |
| Tyr -7 | - | expression tag |
| Lys -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Pro 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 1012 | Arg 12 | variant |
| Thr 1054 | Cys 54 | engineered mutation |
| Thr 1074 | Ala 74 | engineered mutation |
| Ala 1097 | Cys 97 | engineered mutation |
| Arg 1137 | Ile 137 | variant |
| Gly 1162 | - | linker |
| Ser 1163 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 1000 | - | linker |
| Ser 1001 | - | linker |
| Ala 286 | Gly 286 | engineered mutation |
| Gly 2000 | - | expression tag |
| Arg 2001 | - | expression tag |
| Pro 2002 | - | expression tag |
| Leu 2003 | - | expression tag |
| Glu 2004 | - | expression tag |
| Val 2005 | - | expression tag |
| Leu 2006 | - | expression tag |
| Phe 2007 | - | expression tag |
| Gln 2008 | - | expression tag |
| Gly 2009 | - | expression tag |
| Pro 2010 | - | expression tag |
| His 2011 | - | expression tag |
| His 2012 | - | expression tag |
| His 2013 | - | expression tag |
| His 2014 | - | expression tag |
| His 2015 | - | expression tag |
| His 2016 | - | expression tag |
| His 2017 | - | expression tag |
| His 2018 | - | expression tag |
| His 2019 | - | expression tag |
| His 2020 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 60023.4 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2239.1 | |
| All* | Total formula weight | 62262.6 |
