6M9T
Crystal structure of EP3 receptor bound to misoprostol-FA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Prostaglandin E2 receptor EP3 subtype, Endolysin chimera | polymer | 537 | 60023.4 | 1 | UniProt (P43115) UniProt (P00720) Pfam (PF00001) Pfam (PF00959) | Homo sapiens (Human) | PGE2 receptor EP3 subtype,PGE2-R,Prostanoid EP3 receptor,Lysis protein,Lysozyme,Muramidase |
| 2 | B (A) | (11alpha,12alpha,13E,16S)-11,16-dihydroxy-16-methyl-9-oxoprost-13-en-1-oic acid | non-polymer | 368.5 | 1 | Chemie (J9P) PubChem (18647288) PubChem (35021740) PubChem (35021746) PubChem (40552772) PubChem (53731010) PubChem (56846193) PubChem (59951373) PubChem (70634661) PubChem (4206) PubChem (84065) PubChem (91573643) PubChem (98618985) PubChem (101656448) PubChem (124305287) PubChem (124389596) PubChem (5353781) PubChem (6436406) PubChem (126968653) PubChem (9820635) PubChem (9976324) PubChem (139593374) | |||
| 3 | C, D, E, F (A) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 4 | G (A) | (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate | non-polymer | 356.5 | 1 | Chemie (OLC) PubChem (25021708) PubChem (46840179) PubChem (54294271) PubChem (54294270) PubChem (33022) PubChem (5283468) PubChem (5364833) PubChem (11451146) PubChem (12178130) | |||
| 5 | H, I, J, K (A) | OLEIC ACID | non-polymer | 282.5 | 4 | Chemie (OLA) PubChem (965) PubChem (445639) PubChem (637517) | |||
| 6 | L (A) | water | water | 18.0 | 5 | Chemie (HOH) |
Sequence modifications
A: 2 - 259 (UniProt: P43115)
A: 1002 - 1161 (UniProt: P00720)
A: 273 - 353 (UniProt: P43115)
| PDB | External Database | Details |
|---|---|---|
| Val -11 | - | expression tag |
| Phe -10 | - | expression tag |
| Ala -9 | - | expression tag |
| Asp -8 | - | expression tag |
| Tyr -7 | - | expression tag |
| Lys -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Pro 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 1012 | Arg 12 | variant |
| Thr 1054 | Cys 54 | engineered mutation |
| Thr 1074 | Ala 74 | engineered mutation |
| Ala 1097 | Cys 97 | engineered mutation |
| Arg 1137 | Ile 137 | variant |
| Gly 1162 | - | linker |
| Ser 1163 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 1000 | - | linker |
| Ser 1001 | - | linker |
| Ala 286 | Gly 286 | engineered mutation |
| Gly 2000 | - | expression tag |
| Arg 2001 | - | expression tag |
| Pro 2002 | - | expression tag |
| Leu 2003 | - | expression tag |
| Glu 2004 | - | expression tag |
| Val 2005 | - | expression tag |
| Leu 2006 | - | expression tag |
| Phe 2007 | - | expression tag |
| Gln 2008 | - | expression tag |
| Gly 2009 | - | expression tag |
| Pro 2010 | - | expression tag |
| His 2011 | - | expression tag |
| His 2012 | - | expression tag |
| His 2013 | - | expression tag |
| His 2014 | - | expression tag |
| His 2015 | - | expression tag |
| His 2016 | - | expression tag |
| His 2017 | - | expression tag |
| His 2018 | - | expression tag |
| His 2019 | - | expression tag |
| His 2020 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 60023.4 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2239.1 | |
| All* | Total formula weight | 62262.6 |
