6M6M

The crystal structure of glycosidase mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beta-glucosidase	polymer	447	49359.1	2	UniProt (D2PL27) Pfam (PF00232)	Kribbella flavida (strain DSM 17836 / JCM 10339 / NBRC 14399)
2	C, D (A, B)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	2	Chemie (TRS)
3	E, F (A, B)	water	water		18.0	1195	Chemie (HOH)

Sequence modifications

A, B: 1 - 446 (UniProt: D2PL27)

PDB	External Database	Details
Phe 0	-	expression tag
Phe 248	Ile 248	engineered mutation
Arg 410	Tyr 410	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	98718.1
Non-Polymers*	Number of molecules	2
	Total formula weight	244.3
All*	Total formula weight	98962.4

*Water molecules are not included.