6M6M
The crystal structure of glycosidase mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.734, 95.432, 93.382 |
Unit cell angles | 90.00, 90.76, 90.00 |
Refinement procedure
Resolution | 32.634 - 2.070 |
R-factor | 0.1354 |
Rwork | 0.134 |
R-free | 0.17820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ta9 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.875 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.110 |
High resolution limit [Å] | 2.070 | 2.070 |
Number of reflections | 342172 | 342172 |
<I/σ(I)> | 27.6 | |
Completeness [%] | 99.9 | |
Redundancy | 3.7 | |
CC(1/2) | 0.990 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCl (pH 8.5), 0.2 M Magnesium chloride, PEG 8000 (20%, w/v) |