6M55

Crystal structure of the E496A mutant of HsBglA in complex with 4-galactosyllactose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, D)	Beta-galactosidase-like enzyme	polymer	542	60452.9	2	UniProt (Q564N5) Pfam (PF00232)	Hamamotoa singularis	GH1 beta-glucosidase
2	C, D (C, F)	beta-D-galactopyranose-(1-4)-beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	2	In PDB GlyTouCan (G33559CW)
3	E, F (B, E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	G, H (A, D)	alpha-D-mannopyranose	non-polymer		180.2	2	Chemie (MAN)
5	I, J, K, L (D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
6	M, N (A, D)	water	water		18.0	3	Chemie (HOH)

Sequence modifications

A, D: 55 - 594 (UniProt: Q564N5)

PDB	External Database	Details
Ala 496	Glu 496	engineered mutation
His 595	-	expression tag
His 596	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	120905.9
Branched	Number of molecules	4
	Total formula weight	1857.7
Non-Polymers*	Number of molecules	6
	Total formula weight	1245.1
All*	Total formula weight	124008.7

*Water molecules are not included.