6M55
Crystal structure of the E496A mutant of HsBglA in complex with 4-galactosyllactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 184.686, 67.116, 117.585 |
Unit cell angles | 90.00, 128.54, 90.00 |
Refinement procedure
Resolution | 45.210 - 3.000 |
R-factor | 0.266 |
Rwork | 0.265 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gxp |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.990 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.161 | 0.564 |
Rmeas | 0.176 | 0.625 |
Rpim | 0.069 | 0.266 |
Number of reflections | 24658 | 1795 |
<I/σ(I)> | 7.27 | 1.57 |
Completeness [%] | 96.7 | 96.2 |
Redundancy | 6.1 | 4.9 |
CC(1/2) | 0.993 | 0.912 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M SPG Buffer pH 7.0, 27% (w/v) PEG 1500 |