6M3D
X-ray crystal structure of tandemly connected engrailed homeodomains (EHD) with R53A mutations and DNA complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | DNA (5'-D(*TP*AP*AP*TP*CP*CP*TP*AP*AP*TP*CP*C)-3') | polymer | 12 | 3581.4 | 1 | synthetic construct | ||
2 | B | DNA (5'-D(*GP*GP*AP*TP*TP*AP*GP*GP*AP*TP*TP*A)-3') | polymer | 12 | 3741.5 | 1 | synthetic construct | ||
3 | C | Segmentation polarity homeobox protein engrailed,Segmentation polarity homeobox protein engrailed | polymer | 148 | 17344.6 | 1 | UniProt (P02836) Pfam (PF00046) In PDB | Drosophila melanogaster (Fruit fly) | |
4 | A | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
5 | water | water | 18.0 | 207 | Chemie (HOH) |
Sequence modifications
C: 22 - 81 (UniProt: P02836)
C: 87 - 146 (UniProt: P02836)
PDB | External Database | Details |
---|---|---|
Met 1 | - | initiating methionine |
Gly 2 | - | expression tag |
Ser 3 | - | expression tag |
Ser 4 | - | expression tag |
His 5 | - | expression tag |
His 6 | - | expression tag |
His 7 | - | expression tag |
His 8 | - | expression tag |
His 9 | - | expression tag |
His 10 | - | expression tag |
Ala 11 | - | expression tag |
Ile 12 | - | expression tag |
Glu 13 | - | expression tag |
Asp 14 | - | expression tag |
Leu 15 | - | expression tag |
Tyr 16 | - | expression tag |
Phe 17 | - | expression tag |
Gln 18 | - | expression tag |
Ser 19 | - | expression tag |
Pro 20 | - | expression tag |
Gly 21 | - | expression tag |
Lys 72 | Gln 503 | engineered mutation |
Ala 75 | Arg 506 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 82 | - | linker |
Gly 83 | - | linker |
Gly 84 | - | linker |
Gly 85 | - | linker |
Gly 86 | - | linker |
Lys 137 | Gln 503 | engineered mutation |
Ala 140 | Arg 506 | engineered mutation |
Gly 147 | - | expression tag |
Thr 148 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 24667.4 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 23.0 | |
All* | Total formula weight | 24690.4 |