6M3D

X-ray crystal structure of tandemly connected engrailed homeodomains (EHD) with R53A mutations and DNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA (5'-D(*TP*AP*AP*TP*CP*CP*TP*AP*AP*TP*CP*C)-3')	polymer	12	3581.4	1		synthetic construct
2	B (B)	DNA (5'-D(*GP*GP*AP*TP*TP*AP*GP*GP*AP*TP*TP*A)-3')	polymer	12	3741.5	1		synthetic construct
3	C (C)	Segmentation polarity homeobox protein engrailed,Segmentation polarity homeobox protein engrailed	polymer	148	17344.6	1	UniProt (P02836) Pfam (PF00046)	Drosophila melanogaster (Fruit fly)
4	D (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	E, F, G (A, B, C)	water	water		18.0	207	Chemie (HOH)

Sequence modifications

C: 22 - 81 (UniProt: P02836)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ala 11	-	expression tag
Ile 12	-	expression tag
Glu 13	-	expression tag
Asp 14	-	expression tag
Leu 15	-	expression tag
Tyr 16	-	expression tag
Phe 17	-	expression tag
Gln 18	-	expression tag
Ser 19	-	expression tag
Pro 20	-	expression tag
Gly 21	-	expression tag
Lys 72	Gln 503	engineered mutation
Ala 75	Arg 506	engineered mutation

C: 87 - 146 (UniProt: P02836)

PDB	External Database	Details
Gly 82	-	linker
Gly 83	-	linker
Gly 84	-	linker
Gly 85	-	linker
Gly 86	-	linker
Lys 137	Gln 503	engineered mutation
Ala 140	Arg 506	engineered mutation
Gly 147	-	expression tag
Thr 148	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	24667.4
Non-Polymers*	Number of molecules	1
	Total formula weight	23.0
All*	Total formula weight	24690.4

*Water molecules are not included.