6M3D
X-ray crystal structure of tandemly connected engrailed homeodomains (EHD) with R53A mutations and DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.798, 61.437, 39.976 |
| Unit cell angles | 90.00, 106.45, 90.00 |
Refinement procedure
| Resolution | 38.170 - 1.600 |
| R-factor | 0.190614050402 |
| Rwork | 0.188 |
| R-free | 0.23586 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hdd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.005 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.170 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.025 | 0.252 |
| Rmeas | 0.034 | 0.338 |
| Number of reflections | 24423 | 1183 |
| <I/σ(I)> | 15.7 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.999 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 8% (w/v) PEG 4000, 0.1 M sodium acetate |
