6M23

Overall structure of KCC2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Solute carrier family 12 member 5	polymer	1129	125295.6	2	UniProt (Q9H2X9) Pfam (PF00324) Pfam (PF03522)	Homo sapiens (Human)	Electroneutral potassium-chloride cotransporter 2,K-Cl cotransporter 2,hKCC2,Neuronal K-Cl cotransporter
2	C, D, E, F, G... (C, D, E, F, G...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	6	In PDB GlyTouCan (G42666HT)
3	I, L (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
4	J, M (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	K, N (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
6	O, P (A, B)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A, B: 1 - 1116 (UniProt: Q9H2X9)

PDB	External Database	Details
Met -12	-	initiating methionine
Ala -11	-	expression tag
Asp -10	-	expression tag
Tyr -9	-	expression tag
Lys -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Lys -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	250591.2
Branched	Number of molecules	6
	Total formula weight	2546.4
Non-Polymers*	Number of molecules	6
	Total formula weight	591.5
All*	Total formula weight	253729.2

*Water molecules are not included.