6M0W
Crystal structure of Streptococcus thermophilus Cas9 in complex with the AGAA PAM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | RNA (72-MER) | polymer | 72 | 23227.8 | 1 | Streptococcus thermophilus | ||
| 2 | B (C) | DNA (28-MER) | polymer | 28 | 8496.5 | 1 | Streptococcus thermophilus | ||
| 3 | C (D) | DNA (5'-D(*AP*AP*AP*GP*AP*AP*GP*C)-3') | polymer | 8 | 2468.7 | 1 | Streptococcus thermophilus | ||
| 4 | D (A) | CRISPR-associated endonuclease Cas9 1 | polymer | 1122 | 129646.9 | 1 | UniProt (Q03LF7) | Streptococcus thermophilus LMD-9 | Cas9** |
| 5 | E, F, G, H, I (B, D, A) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 6 | J, K, L (B, C, A) | water | water | 18.0 | 19 | Chemie (HOH) |
Sequence modifications
A: 2 - 1121 (UniProt: Q03LF7)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Gly 1 | - | expression tag |
| Ala 599 | His 599 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 163839.9 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 121.5 | |
| All* | Total formula weight | 163961.4 |
