6M0W

Crystal structure of Streptococcus thermophilus Cas9 in complex with the AGAA PAM

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004519	molecular_function	endonuclease activity
A	0043571	biological_process	maintenance of CRISPR repeat elements
A	0046872	molecular_function	metal ion binding
A	0051607	biological_process	defense response to virus

Functional Information from PDB Data

site_id	AC1
Number of Residues	1
Details	binding site for residue MG B 101

Chain	Residue
B	G40

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site_id	AC2
Number of Residues	5
Details	binding site for residue MG A 1201

Chain	Residue
A	ASP598
A	ALA599
A	ASN622
C	DC11
C	DC12

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site_id	AC3
Number of Residues	1
Details	binding site for residue MG A 1202

Chain	Residue
A	LYS848

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site_id	AC4
Number of Residues	3
Details	binding site for residue MG A 1203

Chain	Residue
C	HOH101
A	ASP824
A	SER961

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: For RuvC-like nuclease domain => ECO:0000255|HAMAP-Rule:MF_01480

Chain	Residue	Details
A	ASP9

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton acceptor for HNH nuclease domain => ECO:0000255|HAMAP-Rule:MF_01480

Chain	Residue	Details
A	ALA599

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site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01480

Chain	Residue	Details
A	GLU513
A	HIS738
A	ASP9
A	GLU509

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