6LZ9

t8E4 antibody Fab complexed with the active form of HGF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Hepatocyte growth factor	polymer	108	12280.6	1	UniProt (P14210) Pfam (PF00051)	Homo sapiens (Human)	Hepatopoietin-A,Scatter factor,SF
2	B (B)	Hepatocyte growth factor	polymer	242	27107.4	1	UniProt (P14210) Pfam (PF00089)	Homo sapiens (Human)	Hepatopoietin-A,Scatter factor,SF
3	C (H)	Heavy chain of t8E4 Fab fragment	polymer	223	24075.0	1		Mus musculus
4	D (L)	Light chain of t8E4 Fab fragment	polymer	214	23847.2	1		Mus musculus
5	E, F, G (B, H, L)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A: 388 - 494 (UniProt: P14210)

PDB	External Database	Details
Ser 387	-	expression tag
Gln 402	Asn 402	engineered mutation
Gly 476	Thr 476	engineered mutation
Ile 491	Lys 491	engineered mutation
Glu 492	Gln 492	engineered mutation
Gly 493	Leu 493	engineered mutation

B: 495 - 728 (UniProt: P14210)

PDB	External Database	Details
Ser 561	Cys 561	engineered mutation
Gln 566	Asn 566	engineered mutation
Gln 653	Asn 653	engineered mutation
Arg 729	-	expression tag
Leu 730	-	expression tag
Glu 731	-	expression tag
Asn 732	-	expression tag
Leu 733	-	expression tag
Tyr 734	-	expression tag
Phe 735	-	expression tag
Gln 736	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	87310.2
All*	Total formula weight	87310.2

*Water molecules are not included.